transwell inserts Search Results


filter pore size transwell insert  (Greiner Bio)
96
Greiner Bio filter pore size transwell insert
Filter Pore Size Transwell Insert, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
filter pore size transwell insert - by Bioz Stars, 2026-03
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matrigel coated 24 well plate cell culture inserts  (Greiner Bio)
96
Greiner Bio matrigel coated 24 well plate cell culture inserts
Matrigel Coated 24 Well Plate Cell Culture Inserts, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matrigel coated 24 well plate cell culture inserts/product/Greiner Bio
Average 96 stars, based on 1 article reviews
matrigel coated 24 well plate cell culture inserts - by Bioz Stars, 2026-03
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pore size pet thincert transwell inserts  (Greiner Bio)
96
Greiner Bio pore size pet thincert transwell inserts
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Pore Size Pet Thincert Transwell Inserts, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pore size pet thincert transwell inserts/product/Greiner Bio
Average 96 stars, based on 1 article reviews
pore size pet thincert transwell inserts - by Bioz Stars, 2026-03
96/100 stars
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12-well transwell plate inserts  (Becton Dickinson)
90
Becton Dickinson 12-well transwell plate inserts
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
12 Well Transwell Plate Inserts, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/12-well transwell plate inserts/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
12-well transwell plate inserts - by Bioz Stars, 2026-03
90/100 stars
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costar transwell insert  (Corning Life Sciences)
90
Corning Life Sciences costar transwell insert
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Costar Transwell Insert, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/costar transwell insert/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
costar transwell insert - by Bioz Stars, 2026-03
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basement membrane matrix-coated upper sides of transwell inserts corning  (Corning Life Sciences)
90
Corning Life Sciences basement membrane matrix-coated upper sides of transwell inserts corning
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Basement Membrane Matrix Coated Upper Sides Of Transwell Inserts Corning, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/basement membrane matrix-coated upper sides of transwell inserts corning/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
basement membrane matrix-coated upper sides of transwell inserts corning - by Bioz Stars, 2026-03
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transwell insert with pores  (Greiner Bio)
90
Greiner Bio transwell insert with pores
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Transwell Insert With Pores, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transwell insert with pores/product/Greiner Bio
Average 90 stars, based on 1 article reviews
transwell insert with pores - by Bioz Stars, 2026-03
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polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert, sterile  (Corning Life Sciences)
90
Corning Life Sciences polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert, sterile
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Polytetrafluoroethylene Filters Transwell Transwell® With Pore Polyester Membrane Insert, Sterile, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert, sterile/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
polytetrafluoroethylene filters transwell transwell® with pore polyester membrane insert, sterile - by Bioz Stars, 2026-03
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transwell insert pet membrane corning 3464  (Corning Life Sciences)
90
Corning Life Sciences transwell insert pet membrane corning 3464
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Transwell Insert Pet Membrane Corning 3464, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transwell membranes  (Becton Dickinson)
90
Becton Dickinson transwell membranes
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Transwell Membranes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transwell insert 8-um pores  (Collaborative Research Inc)
90
Collaborative Research Inc transwell insert 8-um pores
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Transwell Insert 8 Um Pores, supplied by Collaborative Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transwell insert 8-um pores/product/Collaborative Research Inc
Average 90 stars, based on 1 article reviews
transwell insert 8-um pores - by Bioz Stars, 2026-03
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matrigel matrix basement membrane- precoated transwell insert  (Corning Life Sciences)
90
Corning Life Sciences matrigel matrix basement membrane- precoated transwell insert
Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one <t>transwell</t> insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.
Matrigel Matrix Basement Membrane Precoated Transwell Insert, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matrigel matrix basement membrane- precoated transwell insert/product/Corning Life Sciences
Average 90 stars, based on 1 article reviews
matrigel matrix basement membrane- precoated transwell insert - by Bioz Stars, 2026-03
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Image Search Results


Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one transwell insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.

Journal: Gut Microbes

Article Title: SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella -containing vacuoles in vivo

doi: 10.1080/19490976.2021.1973836

Figure Lengend Snippet: Altered population dynamic in vivo and enterocyte egress in vitro of SPI2 T3SS mutant S . Typhimurium. (a and b) violin plots to illustrate the tag sequence identity between intraepithelial wt and Δ sseB S . Typhimurium strains and total liver (a) or spleen (b) tissue from 4 (wt) or 6 (Δ sseB ) animals from one litter. This method visualizes the presence/absence of sequence tags in each organ in respect to the total spectrum of sequence tags found in this animal at day 4 p.i. with an equal mixture of all 22 strains in a total of 10 3 CFU bacteria. Significance was calculated with the Student’s t-test. Median (dashed line), quartile (dotted line) and p -values are shown. (c) Bray-Curtis Index was substracted from 1 for comparison of the similarity between the spectrum of intraepithelial S . Typhimurium strains and the spectrum of S . Typhimurium strains in liver and spleen tissue between wt and Δ sseB S . Typhimurium. (d) number of wt, Δ sseB and Δ ssaN as well as complemented Δ sseB (p sseB ) and Δ ssaN (p ssaN ) S . Typhimurium that egressed at the basolateral side from apically infected polarized intestinal epithelial m-IC cl2 cells within 30 min. Individual data, median, and p -values are indicated. (e) egress rate (%) calculated as total egressed bacteria/intraepithelial bacteria/x100 (see Fig. S1JK). Each data point represents one transwell insert of in total 6–9; data were generated in three independent experiments. One-way ANOVA with Dunnett’s multiple comparisons test. individual data, median, and p -values are indicated. (f) cartoon illustrating the observed block in the transepithelial transport of SCVs generated by SPI2 mutant S . Typhimurium in vivo.

Article Snippet: Cells were seeded on 3 µm pore size PET ThinCert transwell inserts (Greiner Bio-one, Kremsmünster, Austria) and cultured for 10–12 days with medium change every other day to obtain a covalent layer of polarized epithelial cells.

Techniques: In Vivo, In Vitro, Mutagenesis, Sequencing, Infection, Generated, Blocking Assay